响应面法优化提取杨梅果渣中矢车菊-3-葡萄糖苷的研究

Optimizing extraction of cyanidin-3-glucoside from bayberry pomace by response surface methodology

摘要: 以杨梅果渣为原料,研究矢车菊-3-葡萄糖苷的提取条件。在单因素实验的基础上,通过Box-Behnken响应面优化杨梅果渣矢车菊-3-葡萄糖苷的提取工艺,得到最佳工艺参数为:乙醇浓度53%,提取温度67℃,提取时间67min。经回归分析表明:回归方程的R2=0.9983,R2Adj=0.9826,预测值为5.32mg/g。经验证,在最佳提取条件下,矢车菊-3-葡萄糖苷的得率为5.25mg/g,回归模型预测值与实测值的相对误差<1%。

Abstract: The optimum extraction condition of cyanidin-3-glucoside from bayberry pomace was studied.According to the results of single factor experiment, the extraction conditions were optimized with the Box-Behnken response surface method (RSM) .The best extraction conditions were obtained as follows:ethanol concentration was 53%, extraction temperature was 67℃, and extraction time was 67min.The regressive analysis indicated that R2=0.9983, R2Adj=0.9826 in the equation, and the estimating data was 5.32mg/g.As was validated that the extraction rate of cyanidin-3-o-glucoside from bayberry pomace was 5.25mg/g and the relative error between the estimating data of regressive model and actual data was less than 1% under the best extraction conditions.