预处理对干燥罗非鱼片蛋白质变性的影响及优化

Effect of pretreatment on the protein denaturation of dried tilapia fillets

摘要: 以Ca2+-ATPase活性为指标,采用响应面优化分析,研究了预处理试剂丙二醇、丙三醇和NaCl的添加量对干燥罗非鱼片蛋白质变性的影响,得到较优预处理配方为丙二醇2%+丙三醇3%+NaCl1%。采用优化后的预处理配方对罗非鱼片进行预处理,分析干燥罗非鱼片蛋白质变性和组织结构的变化情况,结果表明,经过预处理的干燥罗非鱼片Ca2+-ATPase活性和盐溶性蛋白溶解度有了明显提高,干制样品的组织结构得到明显的改善,并且干燥温度对干燥罗非鱼片的蛋白质变性有显著影响,温度越高,变性程度越严重,组织结构破坏也越严重。

Abstract: Ca2+-ATPase activity as the indicator to reflect the relativity among propylene glycol, glycerol and sodium chloride. Response surface methodology was used to optimize the pretreatment conditions with the result:propylene glycol 2%+glycerol 3%+NaCl 1%. The results showed that pretreatment improved the remains of Ca2+-ATPase activity and salt-solubility of myofibrillar protein obviously. And the tissue structure was improved obviously. Drying temperature could influence protein denaturation obviously. The degeneration was more serious and the organization structure was damaged more seriously under the higher temperature.