羟自由基氧化系统对体外模拟体系中NDMA合成反应的影响
Effect of hydroxyl radical oxidizing systems on formation of NDMA in the simulated system
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摘要: 为了了解不同条件下羟自由基氧化系统对二甲基亚硝胺(NDMA)形成的影响,从而进一步了解肉品氧化与亚硝胺形成之间的关系。本实验在体外模拟系统中,研究了不同浓度过氧化氢/铁离子/抗坏血酸(H2O2/Fe/Asc)氧化体系以及反应时间和反应温度对亚硝酸钠(NaNO2)和二甲胺(DMA)反应生成NDMA的影响。实验结果显示:H2O2浓度增大,NDMA的生成量显著减小(p<0.05),当H2O2浓度为5mmol/L时对NDMA的抑制率可达97.5%。FeCl3浓度变化对NDMA生成量的影响可能与体系中H2O2的浓度有关。反应温度低于40℃时NDMA生成量没有明显变化,且氧化体系中H2O2和FeCl3的浓度对NDMA的生成量没有显著影响。80℃时NDMA的生成量极显著增加(p<0.05),且氧化体系中H2O2和FeCl3的浓度的变化对NDMA生成量的影响也极显著(p<0.01)。氧化体系的条件改变对NDMA的形成有显著的影响。Abstract: To understand the effect of hydroxyl radical oxidizing systems on the formation of N - nitroso -dimethylamine (NDMA) . The effect on the reaction of sodium nitrite (NaNO2) and dim ethylamine (DMA) of different conditions including concentrations of H2O2and FeCl3, time and temperature were investigated. The results indicated that the content of NDMA decreased with the increase of H2O2 concentration, when the H2O2concentration was 5mmol/L, the inhibition ratio reached to 97. 5%. The effect of FeCl3on the NDMA content in the system may depend on the H2O2concentration. The content of NDMA was no significant change below 40℃, but the content of NDMA markedly increased at 80℃ (p<0. 01) . No significant effect of the concentration of H2O2 and FeCl3on the content of NDMA was observed. Or of the concentration of H2O2 and FeCl3 (p<0. 01) in the HRGS. The change of oxidation system conditions had significant effect on the formation of NDMA.
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