Abstract: Lysozyme, as a natural and safe preservative, is widely used in the processing and storage during the transportation of aquatic products. This paper briefly described the physical and chemical properties, antibacterial properties and its function mechanism of lysozyme. Discussed the safety of the modified lysozyme, the optimum condition by using it, the effect of purification of lysozyme etc. The application status of the lysozyme in the refrigeration and packaging, the application researched about the complex biological fresh-keeping agents worldwide and the combination with the ultrahigh pressure treatment and modified atmosphere packaging (MAP) were reviewed. Finally, prospected about the combination of lysozyme with new preservative technologies, such as environmental management system (EMS) , irradiation treatment and manosonication (MS) treatments, were given an overview.