皂化-硅胶柱层析法纯化辣椒红色素

Purification of capsicum red pigments by saponification and silica gel column chromatography

摘要: 探讨了辣椒红色素的纯化工艺,首先采用碱性乙醇皂化法去除辣椒油树脂中的脂肪酸,然后经过硅胶柱层析脱除辣椒红色素中的辣椒碱。实验结果表明,最佳的皂化条件为:NaOH溶液浓度0.8mol/L,固液比1∶15(g/mL),皂化温度50℃,皂化时间2h,乙醇浓度70%(V/V),最佳的硅胶柱层析条件为:上样量1∶40(m样品∶m硅胶),径高比1∶10,洗脱流速1BV/h,得到的辣椒红色素的色价为672。

Abstract: In order to purify the natural capsicum red pigments, alkaline ethanol saponification was taken to remove the fatty acid from the capsicum oleoresin and silica gel column chromatography was used to separate the capsaicin from capsicum red pigments. The results showed that the optimal saponification conditions were as follows :the concentration of NaOH solution was 0.8mol/L , solid -liquid ratio was 1∶15 (g/mL ) , ethanol concentration (V/V ) was 70% , the holding temperature was 50℃ and the saponification time was 2h. The optimum separation was achieved with sample volume at 1∶40, diameter-length ratio at 1∶10 and eluant flow rate at 1BV/h. The color value of purified capsicum red pigments was 672.