Abstract: Objective:The objective of present study is to investigate actomyosin dissociation in post-mortem duck muscles during the storage of 72h at 4℃. Methods:Duck muscles and actomyosin crude extract from postmortem duck muscles were stored at 4℃ for 2, 12, 24, 48, 72h respectively, and actomyosin dissociation was analyzed by Western-blot. Results:It showed that the density of actin bands at 2, 12, 24, 48, 72h were not significantly different (p>0.05) , indicating actomyosin dissociation almost did not occur during postmortem aging , while in the actomyosin extract, the liberated actin increased significantly with the extension of storage time (p<0.05) . Conclusion:Actomyosin dissociation is mainly affected by the intrinsic factors of the duck muscle itself.