真姬菇多酚的微波辅助提取及清除DPPH自由基活性的研究
Study on microwave-assisted extraction and DPPH free radicals scavenging capacity of polyphenols from Hypsizygus marmoreus
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摘要: 采用单因素设计和响应面设计法对真姬菇多酚的微波辅助提取工艺进行了研究。结果表明,微波功率、微波时间、提取剂用量对真姬菇多酚提取效果的影响极显著。响应面实验结果表明,三个因素、二次项及微波功率与微波时间的交互项对提取效果的影响均显著。通过回归模型求解得到的最优提取工艺为:70%乙醇水溶液,提取剂用量15mL/g,在微波功率350W,微波时间120s,50℃水浴中提取2h,多酚得率达2.002mg/g。真姬菇多酚的DPPH自由基清除能力显著高于BHA和VC,且在实验浓度范围内,呈明显的量效关系。Abstract: The extraction conditions of polyphenols from Hypsizygus marmoreus by microwave - assisted extraction (MAE) were studied with single factor design and response surface design. The effect of microwave power , microwave time and solvent amount on extraction yield (EY) were significant. ANOVA for response surface quadratic model was made. The results showed that the linear, quadratic term of the three factors and interactive term between microwave power and time were all significant. Optimized extraction conditions were obtained by regression model solution. Optimal parameters were 70% ethanol, solvent amount 15mL/g, microwave power 350W, extraction time 120s, 50℃ hot water, 2h and actual EY under the optimal conditions was 2.002mg/g. Scavenging capacity for DPPH free radicals of phenolic extract from Hypsizygus marmoreus was higher than BHA and V C . Within the test concentration range, a significant dose-effect relationship was found.
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