硒纳豆激酶液体发酵研究

Study on the liquid fermentation of Selem’Nattokinase

摘要: 通过向液体培养基中添加无机硒,利用纳豆菌的发酵作用实现无机硒到有机硒的生物转化,并与纳豆激酶结合,得到硒纳豆激酶。最优发酵条件为:大豆蛋白胨2.5%,葡萄糖2%,Na2SeO310-6mol/L,MgSO40.05%,CaCl20.02%,K2HPO4/KH2PO40.2%/0.1%,培养基pH7,培养温度37℃,接种量2%,培养时间60h。所得硒纳豆激酶酶活达196.81U/mL（Folin-酚法）,其SDS-PAGE凝胶电泳条带的硒含量为0.060μg/g,远高于不含硒发酵液对照样。

Abstract: This study realized the biological transformation form inorganic selenium into organic selenium by liquid fermentation of the Bacillus natto, and obtained Selem’Nattokinase. Optimal fermentation conditions were 2.5% soy peptone, 2% glucose, 10-6 mol/L Na2SeO3, 0.05% MgSO4, 0.02% CaCl2, 0.2% /0.1% K2 HPO4/KH2PO4, pH7.0, fermentation temperature 37℃ , inoculum size 2% , fermentation time 60h. Under these conditions, the activity of the Selem’Nattokinase reached 196.81U/mL (Folin-phenol method) , and the selenium content of its SDS-PAGE protein band was 0.060μg/g.