固相萃取-高效液相色谱-荧光检测方便面中的苯并（a）芘

doi:10.13386/j.issn1002-0306.2013.08.018

固相萃取-高效液相色谱-荧光检测方便面中的苯并（a）芘

Determination of Benzo (a) pyrene in instant noodles by solid phase extraction-high performance liquid chromatography

摘要

摘要: 采用固相萃取-高效液相色谱-荧光法（HPLC-FLD）测定方便面中苯并（a）芘的含量。根据不同的食品材质对样品提取条件进行优化,采用HiCapt-Benzo苯并（a）芘专用固相萃取柱净化,反相C18（4.6mm×250mm,5μm）色谱柱分离,外标法定量。苯并（a）芘在1.0～200μg/L浓度范围内线性关系良好,相关系数R2=0.9998。本方法的定量限（S/N≥10）在方便面调料中的调味油酱料包为0.5μg/kg,在调味粉料包、菜肉包和面饼都为0.1μg/kg;添加浓度为1.0～20μg/kg时,回收率范围为78.1%～96.7%,相对标准偏差（RSD）0.97%～6.23%（n=6）。该方法前处理简单,分析周期短,具有良好的灵敏度和精密度,适用于方便面中苯并（a）芘的测定。

Abstract: This thesis developed a HPLC method for Quick determination of the benzo (a) pyrene (BaP) level in the instant noodles with fluorescence detector. These samples were treated by the HiCapt-Benzo solid phase extraction (SPE ) column, and then separated by the C18 revered -phase column (4.6mm ×250mm , 5μm) , quantified with the external standard method. It was showed a good linear relationship at a rang of 1.0²00μg/L, R2=0.9998. The limit of detection was 0.5μg/kg in the flavored oil and 0.1μg/kg in the noodles. Linear plots were obtained between 1.0μg/L and 200μg/L. The recovery was 78.1%⁹6.7%, and RSD (n=6) was 0.97%⁶.23%. The result showed that the method was sensitive, accurate, convenient and quick, which was suitable for the B (a) P determination in the instant noodles.