Abstract: Acrylamide (AA) is a toxic compound that could lead the damage of DNA in cells. In present paper, it was mainly discussed the protective effect of five antioxidants (puerarin, luteolin, genistein, quercetin, and naringenin) on AA-induced oxidative injury and kidney damage. Fifty-six male Balb/c mice were randomly divided into seven groups, Control group, AA group, five antioxidant groups. The oxidative injury and kidney injury were evaluated by the indexes of serum , including glutathione (GSH) , superoxide dismutase (SOD) , malondialdehyde (MDA) and serurn urea nitrogen (BUN) . The results showed that compared with AA group, antioxidants groups could increase the content of GSH and SOD activity and decrease the levels of MDA and BUN in the serum of the mice significantly. Genistein had significant protective effects on AA-induced oxidative injury and kidney injury was most obviously.