薰衣草精油对HepG2细胞的抗增殖作用研究

doi:10.13386/j.issn1002-0306.2013.08.008

薰衣草精油对HepG2细胞的抗增殖作用研究

Study on anti proliferation effect of essential oil from the lavender on HepG2 cells

摘要

摘要: 研究了薰衣草精油对HepG2细胞的生长抑制作用。用溴化二苯偶氮盐（MTT）法观察薰衣草精油和20%的薰衣草精油含药血清对HepG2的生长抑制作用;HE染色法观察薰衣草精油对HepG2细胞形态学的影响;流式细胞术检测薰衣草精油对HepG2细胞凋亡的影响。结果:薰衣草精油明显抑制HepG2细胞的增殖,并呈浓度依赖性;20%的薰衣草精油血清对HepG2细胞的抑制率与5-氟尿嘧啶血清相比无显著性差异;薰衣草精油处理HepG2后细胞数及体积减少,胞浆和细胞核浓缩;不同质量浓度的薰衣草精油作用HepG2后出现明显的凋亡细胞群及坏死细胞。结果表明,薰衣草精油可以通过诱导细胞凋亡或坏死的方式抑制HepG2细胞的生长。

Abstract: To investigate the growth inhibitory effect of Lavender essential oil on HepG2 cells. The growth inhibitory effect of Lavender essential oil on HepG2 was detected using MTT assay , the morphology of cells was observed with HE staining, and the apoptosis was assayed with the flow cytometry. Results:Lavender essential oil inhibited the growth of HepG2 cells at a concentrate-dependent manner. However, there was no significant difference between the inhibitory ratios of the 20% serum containing Lavender essential oil on HepG2 and that of 5-fluorouracil. The treatment with Lavender essential oil resulted in the reducion of cell number and volume, condensed cytoplasm and nuclei of cells, as well as apoptosis and necrosis of cells. Results showed that Lavender essential oil inhibits the growth of HepG2 cell via the apoptotic and necrotic manner.