产细菌素屎肠球菌E6的特性分析及发酵条件优化

Characteristics and optimization of fermentative condition for bacteriocin producing Enterococcus Faecium E6

摘要: 研究了屎肠球菌E6抑菌活性的生物学特性,并通过响应面法优化其发酵条件。结果表明,屎肠球菌E6产蛋白质类细菌素,能够抑制单增李斯特氏菌、大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌生长,在pH3.0～7.0条件下有明显抑菌活性,60～121℃热处理20min后仍具有抑菌活性。通过Box-Behnken实验设计优化屎肠球菌E6发酵条件为培养时间36.0h,培养温度31.0℃,培养基pH5.1。在此条件下,发酵上清液抑菌圈直径可达20.17mm,较优化前提高了27.2%。

Abstract: The characteristics of antimicrobial activity and the fermentative condition of Enterococcus Faecium E6 were discussed.Proteinic bacteriocin produced by E6 showed inhibitory activity against Listeria monocytogenes, Escherichia coli, Staphylococcus aureus and Bacillus subtilis.It showed obviously activity under pH3.0～ 7.0 condition.It was also active when heated at 60～ 121℃ for 20min.The fermentative condition, optimized by Box-Behnken design, was cultured at pH 5.1, temperature of 31.0℃ for 36.0h.Under this condition, the diameter of inhibition zone reached up to 20.17mm, which increased by 27.2% than optimization before.