Abstract: The present study aimed to evaluate the process of ultrasound-assisted enzymatic aqueous extraction of oil from tea seeds. The optimization procedure was performed on different crushing conditions, ultrasound conditions, and enzymes. The optimized parameters were as follows:crushing particle size 0.600mm, ultrasonic power 400W, ultrasonic temperature 40℃, ultrasonic time 40min. The order of enzyme efficiency was Alcalase 2.4L>Cellulase>Protex 7L>Viscozyme L. Under the above optimum conditions, the free oil extraction rate was 59.23%, and the total oil extraction rate was 94.56%.