Abstract: The kernel meal of Jatropha curcas L. was hydrolyzed by papain to prepare small peptides which could be as the raw material for the small peptides feed. With the content of small peptides in enzymolysis products as target, the optimized enzymolysis conditions were determined through orthogonal single-factor experiments and orthogonal experiment as follows:enzyme amount 180U/g, enzymolysis time 36h, solid-liquid ratio 1∶3g/mL, pH 5.5, enzymolysis temperature 50℃. Under these conditions, the content of small peptides in enzymolysis products could reach to 217.42mg/g. Small peptides feed could be directly produced by this technology to improve the utilization ratio of the protein, which was very important for the comprehensive utilization of Jatropha curcas resource and relieving the shortage of protein feed.