毛细管电泳法同时检测南蛇藤中三种黄酮类活性成分

doi:10.13386/j.issn1002-0306.2013.06.006

毛细管电泳法同时检测南蛇藤中三种黄酮类活性成分

Simultaneous determination of three flavoids in Celastrus orbiculatus Thunb. using capillary zone electrophoresis

摘要

摘要: 目的:建立同时测定南蛇藤中芦丁、山奈酚及槲皮素含量的毛细管电泳法,分析结果差异,探讨南蛇藤的药用质量控制方法。方法:采用30mmol·L-1硼酸钠缓冲液（pH=9.0）,20kV分离电压,254nm波长条件下采用毛细管电泳法测定芦丁、山萘酚、槲皮素含量。结果:黄酮类物质芦丁、山萘酚和槲皮素的对照品在12min内实现完全分离,在2.5⁴00μg·mL-1范围内呈良好的线性关系。结论:南蛇藤中黄酮类物质含量丰富,毛细管电泳技术可以同时测定南蛇藤中芦丁、山奈酚与槲皮素,具有较好的准确度和精密性,为临床和科研提供了一种简单快速的检测方法,为南蛇藤的药用质量控制提供了技术方法和理论数据。

Abstract: Objective:A new capillary zone electrophoresis (CZE) method was developed for the simultaneous quantification of rutin, kaempferol and quercetin in Celastrus orbiculatus Thunb. Different results were analysed and the medicinal quality control methods were discussed. Methods:The electrophoretic analysis was performed on 30mmol·L-1 sodium borate solution (pH=9.0) , 20kV electrophoresis voltage and 254nm wavelength. Results:Rutin, kaempferol and quercetin were separated successfully in 12min by CZE, a good linearity between peak area and concentration was found in range of 2.5⁴00μg·mL-1 for three compounds. Conclusion:Flavonoids were abundant in Celastrus orbiculatus Thunb. Simultaneous determination of rutin, kaempferol and quercetin by CZE method had a good accuracy and precision. The method was simple, fast and applicable for clinical and scientific research to determine three flavonoids. This provided a technology method and solid theoretical data for Celastrus orbiculatus Thunb. of medicinal quality control.