红三叶预加工废水中叶绿素的提取及其稳定性研究
Extraction of chlorophyll from red clover advance processing wastewater and its stability study
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摘要: 以红三叶鲜青草预加工废水为原料,研究提取叶绿素的方法。首先,采用醇沉法沉降红三叶预加工废水中的有机物;其次,采用醇提法浸提沉淀物中的叶绿素,并通过单因素及正交实验对提取条件进行了优化。结果显示,从废水中沉降有机物的最佳乙醇浓度为30%,固形物沉降率为39.45%;从沉淀物中浸提叶绿素的最佳条件为乙醇浓度90%、浸提温度60℃、浸提时间6h和料液比1∶25（g/mL）,叶绿素提取率为1.72mg/g。稳定性研究表明,该方法制备的红三叶叶绿素具有良好的耐热性和抗氧化性,在弱酸、弱碱条件下较稳定,Fe2+有助于其稳定性的提高,在光照条件下易发生降解。Abstract: A method to extract chlorophyll from RCPW was reported. Firstly, organics in RCPW was precipitated by ethanol;secondly, chlorophyll in precipitate was extracted by ethanol, the single factor and orthogonal test were designed to optimize extraction parameters. The results indicated that the optimal ethanol concentration to precipitate organics in RCPW was 30%, solid sedimentation rate was 39.45%;the optimal conditions to extract chlorophyll from precipitate were ethanol concentration 90%, extraction temperature 60℃, extraction time 6h and solid-to-liquid ratio 1∶25 (g/mL) , chlorophyll extraction rate was 1.72mg/g. The stability study showed that the red clover chlorophyll extracted by above method was well resistant to heat and oxidant, relatively stable in weak acid or base environment, improved by Fe2+, but easily degraded on light illumination.
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