Abstract: Discussed the preparation calcium citrate using egg shell. The eggshell was calcined at high temperature by high temperature calcination method, so that the CaCO3 contained in the eggshell was completely decomposed to CaO, and then adding citric acid preparation of calcium citrate to the neutralization reaction. High-temperature calcination conditions were investigated, and the best calcination conditions were obtained. The dosage of water, citric acid concentration and reaction temperature were studied through the orthogonal experimental stage in the neutralization reaction, and the experimental results were analyzed by the integrated weighted score and range analysis. The result showed that in the optimal reaction conditions:the dosage of water 50mL, citric acid concentration was 1mol/L, the dosage of citric acid 35mL, neutralization reaction temperature of 70℃. In the optimal reaction conditions, the yield of calcium citrate was 84.36%±0.48%, content of 87.84%±0.51%.