Abstract: In order to develop a new selenium enriched food, Na2SeO3 was used as the selenium source and soybean was chosen as a carrier in the study. After being soaked in the Na2SeO3 solution with a given concentration and temperature for several hours, and then being germinated in the ambient with a given temperature for a period, selenium enriched soybean was generated. The best selenium enrichment technology and the selenium content of the new product were studied in the paper. Result indicated that the best selenium enrichment condition was:Na2SeO3 concentration 80μg/mL, soaking time 8h, soaking temperature 30℃, germinating time 36h, and germinating temperature 25℃. Total selenium content of the selenium enrichment soybean and its isolated protein were 27.31 and 31.95μg/g. This study provided some primary information to the development of selenium enriched food.