氮元素对地木耳脂肪酸的影响
Effect of nitrogen on fatty acids composition of Nostoc commune
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摘要: 采用改进的Bligh-Dyer（mBD）法,气相色谱-质谱联用法（GC-MS）对含氮（BG11）和无氮（BG110）条件下培养的地木耳（Nostoc commune）脂肪酸进行提取、分离和测定。结果显示:两种培养条件下的地木耳脂肪酸都以C16和C18脂肪酸为主,而且不饱和脂肪酸含量均高于50%。无氮培养条件下地木耳总脂肪酸含量为13.29mg/g,饱和脂肪酸占36.91%,不饱和脂肪酸占63.09%,其中C18∶1的含量为15.99%;含氮条件下培养的地木耳总脂肪酸含量为7.72mg/g,饱和脂肪酸占47.91%,不饱和脂肪酸占52.09%,其中C18∶1的含量为1.74%。结论:无氮培养地木耳的脂肪酸含量显著高于含氮培养组（p<0.05）,表明氮源缺失有利于地木耳脂肪酸特别是不饱和脂肪酸的积累。Abstract: Nostoc commune cultivated in two different conditions (with or without nitrogen in medium) were analyzed for their fatty acids composition using a modified Bligh-Dyer method and GC-MS. The results indicated that C16 and C18 were dominant compositions of total fatty acids (TFAs) , among which the contents of unsaturated fatty acids (UFAs) were all above 50% in all tested samples. TFAs concentration of N. commune cultivated in medium without nitrogen (BG110) was 13.29mg/g, while that of saturated fatty acids (SFAs) and UFAs were present at 36.91%, 63.09% respectively. C18：1 was present at 15.99% in particular;TFAs concentration of N. commune cultivated in medium with nitrogen (BG11) was 7.72mg/g, while concentrations of SFAs and UFAs were present at 47.91%, 52.09% respectively. C18∶1 was 1.74%. The present study showed that the contents of TFAs especially UFAs was significantly (p<0.05) higher in BG110 group than in BG11 group. This demonstrated that the absence of nitrogen was propitious to the accumulation of fatty acids especially UFAs.
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