费菜多酚含量的测定及体外抗菌活性研究

doi:10.13386/j.issn1002-0306.2013.05.043

费菜多酚含量的测定及体外抗菌活性研究

Determination of total polyphenols and antimicrobial activity of methanol extract from Sedum aizoon L.

摘要

摘要: 目的:研究药食两用植物费菜不同部位多酚含量及其70%甲醇提取物体外抗菌活性。方法:采用热回流提取法70%甲醇提取费菜,使用福林酚（Folin-Ciocalteu）法对其不同部位多酚含量进行测定;采用牛津杯法和96孔板法测定抑菌圈大小、最小抑菌浓度（MIC）和最小杀菌浓度（MBC）,比较费菜70%甲醇提取物对10种细菌的抑制作用。结果:费菜地上部分和地下部分多酚含量分别为45.59±0.87和96.25±0.47mg/g（以没食子酸量/干材料表示）,地下部分多酚含量是地上的2倍多。费菜70%甲醇提取物对8种常见的细菌均表现出较强的抑菌活性,金黄色葡萄球菌对提取物最为敏感,其MIC和MBC分别为6.25、25mg/mL。

Abstract: Objective:To determine the content of total polyphenols in aboveground part and underground part of Sedum aizoon L. , and to research the antimicrobial activities of the 70% methanol extract from Sedum aizoon L. Methods:The content of total polyphenols was determined by Folin-Ciocalteu colorimetric assay with gallic acid as reference. Heat reflux extraction was used to get 70% methanol extract, and antimicrobial activity was evaluated using the Oxford cup method and 96-well plates to measure the size of the zone of inhibition, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) . Results:The results showed that the contents of total polyphenols in aboveground part and underground part of Sedum aizoon L. were (45. 59±0. 87) mg/g and (96. 25±0. 47) mg/g, respectively. And the 70% methanol extract from Sedum aizoon L. was active against almost all tested microbial species. The Staphylococcus aureus was most inhibited by the methanol extract, the MIC and MBC was 6. 25 and 25mg/mL, respectively.