薰衣草总黄酮的微波提取及其抗氧化性研究

Study on microwave extraction and antioxidant activity of total flavonoid in lavender

摘要: 以伊犁产薰衣草为原料,研究微波辅助萃取法提取薰衣草总黄酮。采用单因素和正交实验确定了最佳提取条件,并通过清除超氧阴离子自由基、DPPH自由基、羟基自由基和抑制亚油酸过氧化等评价体系研究了提取物的抗氧化活性。结果表明:微波对薰衣草总黄酮有较好的提取效果。最佳提取条件是:乙醇体积分数45%、料液比1∶25、微波功率500W、微波时间25min,此条件下提取率为8.16%。提取物有较强的抗氧化活性,且黄酮类物质含量越高,抗氧化活性越强。

Abstract: To study microwave-assisted extraction for flavonoid of lavender produced in YiLi area. The single factor and orthogonal test was used to optimized process technology conditions of extracting flavonoids of lavender. The antioxidant activity of extracts was determined. The results showed that the microwave-assisted had good effect on extracting flavonoids of lavende. The optimum technological conditions:ethanol concentration 45%, ratio of material to liquid 1∶25, microwave power 500W, microwave time 25min. The extracting rate was 8.16%. Research results showed that the extracts of lavender had very strong scavenging ability on superoxide radical, DPPH free radicals and hydroxyl radicals. It had good inhibitory effect to linoleic acid oxidation reaction. Antioxidant activity increased with concentration enhanced.