滇黄精黄酮提取工艺及活性的初步研究

Study on extraction process of flavonoids from Polygonatum kingianum Coll.et Hemsl and antioxidative activities of flavonoids

摘要: 研究了滇黄精中的黄酮类物质的提取工艺和抗氧化性能。用乙醇作为溶剂,采用正交实验对滇黄精黄酮提取条件乙醇浓度、液料比、超声波时间、提取次数进行正交优化,最佳条件为:乙醇浓度为80%、液料比为25∶1、提取时间为60min、提取次数3次,此条件下滇黄精黄酮最大提取率为0.40%;以芦丁为对照,对滇黄精黄酮进行DPPH自由基清除率、还原力测定实验,结果显示其活力高于同浓度的芦丁。实验结果显示滇黄精是一值得深入研究和开发利用的药材资源。

Abstract: The extraction process of flavonoids from Polygonatum kingianum Coll. et Hemsl was investigated by the single factor selecting experiment and orthogonal experiment. The results showed that:alcohol concentration was 80%, liquid-solid ratio was 25∶1, ultrasonic wave extraction time was 60min and number of extraction times was 3. The maximum extraction rate for flavonoid was 0.40%. Contrasted with rutin under the circumstance of the concentration, flavonoid in Polygonatum kingianum had stronger antioxidant effect.