Abstract: The phospholipases, including phospholipase A 1 、A 2 、B 、C and D, that are a complex and crucially important group of enzymes that exit in organism and can specifically hydrolyze the intramolecular ester bond of p-hosphoglycerides releasing different products.Thus, phospholipases are widely used in phosphoglycerides transformation.The extraction of phospholipases from animal organs by the traditional means cannot meet the current industry requirements, however, the study of microbial source phospholipases and the rapid development of gene engineering not only broaden the sources of them but also improve their properties effectively.The paper reviewed the progress in the screening of the high yield strain and phospholipases molecular modification and transformation.