Abstract: The change of the bacterial numbers in goat milk samples at different milking stage were studied in this paper. The results showed that the number of the total bacterial count, coliform, psychrotrophilic bacteria, thermophilic bacteria, proteolytic bacteria and lipolysis bacteria was higher in the pre- milk samples, which was also obviously higher than that of the middle and the end milk samples ( p < 0.05) . These bacterial numbers had reached 3 × 10 6 、1.4 ×10 4 、3.3 × 10 4 、1.3 × 10 3 、1.5 × 10 5 and 1.3 × 10 5 cfu /mL, respectively, the pre- milk samples of the total bacterial count had exceed national standard ( GB19301-2010) for purchase fresh milk in the microbial index.So the pre-milk samples should be abandoned in milking process as far as possible to improve the sanitary quality of raw goat milk.