Abstract: The preparation conditions of VC nanoliposomes suspension prepared by the ethanol injection-high pressure homogenization method were optimized. The results of single factor tests and orthogonal test indicated that VC at the dosage of 160mg, cholesterol to lecithin mass ratio of 1 ∶ 5, Tween80 to lecithin mass ratio of 4 ∶ 5, hydration temperature at 55℃ were optimum parameters. VC nanoliposomes suspension obtained were characterized and the results showed that the average encapsulation efficiency, the average size and polydispersity index ( PDI) were 78.11% , 89.62nm and 0.160, respectively.The proliposomes was obtained from the VC nanolipoaomes suspension by freeze-drying method and its average size and PDI were 121.14nm and 0.195. Additionally, it was found that the stability of both VC nanoliposomes suspension and VC proliposomes decreased with the increasing of storage temperature or time, but the proliposomes was more stable than the nanoliposomes suspension.