羊肚菌菌丝体液体培养富锌条件的优化

doi:10.13386/j.issn1002-0306.2013.03.017

羊肚菌菌丝体液体培养富锌条件的优化

Optimization of the conditions of Morchella esculenta mycelium liquid culture Zinc-riching

摘要

摘要: 通过对蛹虫草（Cordyceps militaris）、大球盖菇（Stropharia rugoso-annulata）、铆钉菇（Gomphidiaceae）、杏鲍菇（Pleurotus eryngii）、美味牛肝菌（Boletus edulis）、羊肚菌（Morehella esculenta）、灵芝（Ganoderma lucidum）和真姬菇（Hypsizygus marmoreus）等食用菌富锌能力的考察,筛选出具有较强的耐锌和富锌能力的羊肚菌做为富锌菌种,并采用液体发酵技术对羊肚菌菌丝体富锌适宜的锌源、锌浓度和发酵条件进行了优化。结果表明:羊肚菌菌丝体富锌适宜的锌源是Zn（CH3COO）2,在100～800mg/L的锌浓度范围内菌丝体都可以生长,菌丝体对锌的最适富集浓度为600mg/L。羊肚菌菌丝体最优富锌条件为:发酵温度25℃,起始pH7,振荡转速150r/min,250mL三角瓶装液50mL,锌添加量为600mg/L,接种量5%（V∶V）培养时间4d时,羊肚菌菌丝体的生物量及含锌量达最高,总富锌率可达23.20%,富集锌的有机化程度为37.71%。

Abstract: The abilities of Zinc-riching of Cordyceps militaris, Stropharia rug-annulata, Gomphidiaceae, Pleurotus eryngii, Boletus edulis, Morehella esculenta, Ganoderma lucidum and Hypsizygus marmoreus were studied. The mycelium liquid fermentation conditions of Zinc-riching of Morchella esculenta were studied. The results showed that Morchella esculenta mycelium had high capacities of Zn-resisting and Zn-accumulating. The optimal Zinc source was Zn ( CH 3 COO) 2 . The strain could grow with Zinc concentration from 100mg /L to 800mg /L. When Zinc added to the liquid medium 600mg /L, the mycelium had the most Zinc absorbance.The optimum Zinc-riching liquid fermentation conditions were as following:fermention temperature 25℃, pH7, stirring speed 150r /min, 250mL shake flash containing 50mL medium, adding Zinc 600mg /L, inoculation size 5% and culture for 4d.Under this condition, the ratio of mycelia Zinc-riching reached a maximum of 23.20%, and the degree of Zinc organic was 37.71%.