转基因玉米Bt176品系特异性环介导等温扩增检测方法的研究

Research of the event-specific loop-mediated isothermal amplification method for detection of transgenic maize Bt176

摘要: 根据转基因玉米品系Bt176外源插入片段与玉米基因组序列设计和筛选品系特异性引物,并对反应体系和反应条件进行优化,最终建立转基因玉米品系Bt176的品系特异性LAMP检测方法。对该方法进行了灵敏度、特异性和稳定性评价。评价结果表明:该方法定性检测低限为0.5%,特异性和稳定性均达到100%。

Abstract: According to the exogenous insert sequences of the transgenic maize event Bt176 connected with the maize genome sequences, the event specific primers were designed and screened as well as the reaction system and the parameters were optimized. An event specific loop-mediated isothermal amplification method for the detection of transgenic maize event Bt176 was established in this paper.Moreover, an evaluation of this method on its specificity, sensitivity and stability was performed.The evaluation results showed the LOD of 0.5% , and both the specificity and stability of 100%.