Abstract: Spinaches were dipped into limonene emulsion solution for 3min, and the effect of limonene emulsion on post-harvest physiology and storage quality of spinaches was evaluated.The results indicated that the membrane permeability was obviously lower than that of the control (p<0.05) , which indicated that membrane of spinach was protected by limonene emulsion.Compared with the control, chlorophyll content was sharply improved by limonene emulsion treatment during the early stage of storage (0 ~ 3d) (p<0.05) .The contents of vitamin C and total soluble solid (TSS) on the 6th storage day were all significantly higher in spinaches treated by limonene emulsion (p<0.05) .No obvious difference was observed in content of titration acid and soluble protein between limonene emulsion treat ones and the controls (p>0.05) .Limonene emulsion could hold the structure of the cell membrane in spinach during the whole storage time (0 ~ 9d) , and improve spinaches' quality during the early stage of storage (0 ~ 6d) .