采后BTH处理及粉红单端孢(Trichothecium roseum)挑战接种对厚皮甜瓜果实苯丙烷代谢活性的诱导
Induction of phenylpropanoid metabolic activity in muskmelon fruit by postharvest BTH treatment and challenge inoculation with Trichothecium roseum
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摘要: 以‘玉金香’甜瓜为试材,用0.1g/LBTH浸泡处理10min,测定BTH处理及T.roseum挑战接种对果实苯丙烷代谢关键酶活性和产物积累的影响。结果表明,BTH处理有效降低了损伤接种T.roseum的病斑直径,提高了果实体内苯丙氨酸解氨酶（PAL）、4-香豆酰-辅酶A连接酶（4CL）的活性,增加了总酚、类黄酮及木质素含量。T.roseum挑战接种可进一步促进上述苯丙烷代谢关键酶活性的提高和产物的积累。由此表明,采后BTH处理可通过诱导厚皮甜瓜果实的苯丙烷代谢来增强果实对采后病害的抗性。Abstract: Muskmelon fruit (Cucumis melo L.cv.Yujinxiang) was dipped at 0.1g/L of BTH for 10min.The efficacy of BTH dipping treatment and challenge inoculation with Trichothecium roseum on the activities of key enzyme of phenylpropanoid pathway and metabolites were investigated.The results showed that BTH treatment significantly decreased lesion diameter of muskmelon fruit inoculated with T.roseum.BTH treatment also enhanced the activities of phenylalanine ammonia-lyase (PAL) and Coumaric acid-coenzyme A ligase (4CL) , and increased the contents of total phenolic compounds, flavonoids and lignin.Moreover, challenge inoculation the BTH-induced fruit with T.roseum significantly increased the activities of key enzyme and contents of metobolites in phenylpropanoid pathway.In a conclusion, postharvest BTH treatment increased disease resistance in harvested muskmelon fruit by activating phenylpropanoid pathway.
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