二次正交旋转组合设计优化香菇废菌棒蛋白质的提取工艺
Optimization of extraction technology for protein in Lentinus edodes waste substrate by quadratic regression rotation-orthogonal combination design
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摘要: 采用二次正交旋转组合设计对香菇废菌棒蛋白质提取工艺进行优化。在单因素实验基础上,选择碱浓度、浸提温度、浸提时间、液固比为自变量,香菇废菌棒蛋白质提取量为检测指标,利用二次正交旋转组合进行四因素五水平的实验设计,并做统计分析,建立数学回归模型。结果表明,所得回归方程达到极显著水平,无失拟因素存在。蛋白质的最佳提取工艺条件为:碱浓度0.11mol/L,浸提温度72℃,浸提时间104min,液固比47:1,在此条件下,香菇废菌棒蛋白质提取量理论值为2.38mg/g,验证实测值为2.42mg/g,与理论值相对误差为0.04mg/g,说明所优化的工艺参数准确可靠、稳定、可行。Abstract: The quadratic regression rotation-orthogonal combination design was applied to optimize the extraction conditions of protein in Lentinus edodes waste substrate.On the basis of single-factor test, the mathematical regression model was established about the dependent variable (extraction yield of protein in Lentinus edodes waste substrates) and independent variables (alkali concentration, extraction temperature, extraction time, ratio of solvent to material) through quadratic regression rotation-orthogonal combination design and stat analysis.The results indicated that the obtained regression equation reached to significant level without any lack of fit factor.The optimum condition of protein extraction was alkali concentration of 0.11mol/L, 72℃, 104min extraction, 47:1 of ratio of solvent to material, and the predictive maximum yield of protein in Lentinus edodes waste substrate was 2.38mg/g.The average validation value was 2.42mg/g.The relative error between them was 0.04mg/g.The optimized technology was stable, viable and feasible.
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