Abstract: The beech wood chip and the porous ceramic are used as yeast carriers, these two kinds of carriers were compared by immobilized effect, and the beer from continuous secondary fermentation by these two kind of carriers compared with the beer from traditional secondary fermentation.The results were as follows:the beech wood chips immobilized yeast were more than the porous ceramic 31.2%, the rate of immobilization was more than 45.4%, the stability of the immobilized yeast was better than porous ceramic.Fermented beer by the two carriers compared with each other, which content of diacetyl was significantly different (p<0.05) , the other indexes had no significant differences (p>0.05) .Flavor substances, for example, acetaldehyde, ethyl formate, ethyl acetate, n-propanol, isobutanol, isoamyl acetate, isoamyl alcohol had significant differences (p<0.05) , the rest of the flavor substances were not significant (p>0.05) .The content of amino acids, tyrosine, leucine were not significant (p>0.05) , the rest of the amino acid contents had significant differences (p<0.05) .