紫外线与氯化锂诱变选育高产乳糖酶米曲霉

Breeding of high β-galactosidase producing Aspergillus Oryzae by mutation with UV and LiCl

摘要: 以产乳糖酶米曲霉（ATCC20423）为出发菌株,通过紫外线和紫外复合氯化锂诱变处理选育高产乳糖酶菌株。利用紫外线辐照诱变得到突变株UV-15-20的乳糖酶活力为114.08U/mL,比出发菌株提高了49.22%;以紫外线复合氯化锂选育得到突变株UV-LiCl-38乳糖酶活力为121.42U/mL,比出发菌株提高了58.82%。传代实验表明两个突变株均具有稳定的遗传性。

Abstract: In order to breed high-yield β-Galactosidase producing strain, Aspergillus Oryzae (ATCC20423) was used as the original strain and mutagenized with Ultraviolet (UV) and UV+lithium chloride (LiCl) , respectively.After mutagenized by UV, the β-Galactosidase activity of UV-15-20 reached 114.08U/mL, which revealed a 49.22% increase compared with the original strain.A mutant UV-LiCl-38 with high β-Galactosidase activity (121.42U/mL) was obtained by use of UV and LiCl, the β-galactosidase activity of this mutant was 58.82% higher than that by the wild-type.UV-15-20 and UV-LiCl-38 had good hereditary stability after several generations.