比色法测定不同产地黑豆皮中总黄酮的含量
Determination of total flavonoids in black soybean seed coat from different regions by colorimetric method
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摘要: 目的:建立黑豆皮中总黄酮的含量测定方法,测定八个不同产地黑豆皮中总黄酮的含量。方法:以芦丁为对照品,采用硝酸铝显色,比色法在510nm处测定不同产地的黑豆皮中总黄酮的含量,并考察了黑豆皮中总黄酮的最佳处理方法,保证测定结果的准确性。结果:芦丁在0.003～0.057mg/mL范围内吸光度与浓度呈良好的线性关系,标准曲线回归方程为:Y=12X-0.0009（R2=0.9999）,平均回收率为104.6%。不同产地黑豆皮中总黄酮的含量在6.873%～17.017%之间。结论:所用方法简便、准确,结果显示:不同产地的黑豆皮中总黄酮含量差异较大,山西和安徽的四种黑豆皮中含量较高。Abstract: Objective:To determine the flavonoids content in black soybean seed coat from eight different regions by colormetric method.Methods:Colormetric method was used to determine the total flavonoid contents in black soybeen seed coat from diferent regions at wavelength 510nm by taking rutin as standard substance, and Al (NO4) 3 as chromogenic reagent.The optimum extraction process of total flavonoids in black soybean seed coat was selected for ensure accuracy of the results.Results:The linear equation was Y=12X-0.0009 (R2=0.9999) , and the calibration curve was linear in the range of 0.003 ~ 0.057mg/mL.The average recovery was 104.6%.The contents of total flavonoids in black soybean seed coat from different regions were 6.873% ~ 17.017%.Conclusion:The method was simple and rapid.The result showed that different origin of the content was quite different, with higher levels of Shanxi and Anhui.
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