超临界CO2反溶剂法制备大豆异黄酮-玉米醇溶蛋白复合纳米颗粒
Preparation of soybean isoflavone loaded zein composite nanoparticles by method of supercritical CO2 anti-solvent technology
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摘要: 通过超临界CO2反溶剂法制备大豆异黄酮-玉米醇溶蛋白复合颗粒。采用原子力显微镜观察发现,荷载大豆异黄酮的玉米醇溶蛋白分子可以形成球形颗粒。以玉米醇溶蛋白的自组装特性为基础,改变玉米醇溶蛋白与大豆异黄酮的比例,能使两者形成的复合颗粒的数量平均粒径不超过200nm,其表面负电荷分布在-22.7-34.8mV之间。通过差示扫描量热法、傅里叶变换红外光谱分析表明,氢键、疏水相互作用是玉米醇溶蛋白与大豆异黄酮形成复合颗粒的主要作用力。Abstract: Soybean isoflavone loaded zein composite nanoparticles were prepared by supercritical CO2 anti-solvent technology. The composite nanoparticles were spherical, as confirmed by atomic force microscope (AFM) . Based on the self-assembly of zein, the average number size of composite particles could be controlled below 200nm with negative surface charge (-22.7 to -34.8mV) , depending on the ratio of zein and soybean isoflavone. Solid state characterization by differential scanning calorimetry (DSC) and fourier transform infrared (FTIR) indicated the hydrogen bond and hydrophobic interaction were thought to be main forces during the formation of soybean isoflavone-zein composite nanoparticles.
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