高产洛伐他汀红曲菌株的诱变选育

doi:10.13386/j.issn1002-0306.2012.24.098

高产洛伐他汀红曲菌株的诱变选育

Breeding of high-lovastatin Monascus strains

摘要

摘要: 以红曲霉菌(Monascus Purpureus)HW为出发菌株,利用氯化锂进行诱变。诱变的最佳氯化锂(1mol/L)用量为200μL。经HPLC对诱变菌株发酵产物中的洛伐他汀进行检测,筛选正突变株,最终筛选到6株正突变菌株。其中三株正突变菌株HW3、HW5和HW6的发酵液中洛伐他汀的含量分别达到了35.94、38.86、30.07mg/L,比原始菌株的产洛伐他汀产量分别提高了214%、239%、169%。结合高产洛伐他汀的三株正突变株菌落形态观察发现:洛伐他汀的生产能力与产红曲色素能力有一定的相关性,其次菌落有无皱褶也可能影响洛伐他汀的生产性能。HW3、HW5、HW6正突变株经10次传代培养,产洛伐他汀的性能分别下降11.8%、14.9%、32.1%,HW3、HW5的性状较为稳定,可深入研究。

Abstract: Using Monascus Purpureus HW as original strain, LiCl mutation breading was conducted for increasing the production of lovastatin. The optimal amount of LiCl (1mol/L) was the 200μL. Six of high-lovastatin mutants were respectively obtained through determination of lovastatin in the broth by HPLC. Among these mutants, the yield of lovastatin by HW3, HW5 and HW6 reached 35.94, 38.86 and 30.07mg/L, which were 214% , 239% and 169% higher than that by the original strain, respectively. And the development of colony morphologies indicated that there was a positive correlation between the capacity of lovastatin and pigment among these mutants. Furthermore, colonies with or without folds also affect the performance of lovastatin production. The yield of lovastatin by HW3, HW5 and HW6 decreased by 11.8% , 14.9% and 32.1% , after subculture for 10 times, respectively. Therefore, the HW3 and HW5 mutants are stable strains that are worth further study.