白藜芦醇分子印迹光子晶体水凝胶膜制备及性能研究

doi:10.13386/j.issn1002-0306.2012.24.087

白藜芦醇分子印迹光子晶体水凝胶膜制备及性能研究

Preparation and characterization of resveratrol molecular imprinted photonic hydrogel

摘要

摘要: 为能快速有效地检测样品中白藜芦醇的含量,以光子晶体为模板,白藜芦醇（RES）为印迹模板分子,甲基丙烯酸（MAA）为功能单体,乙二醇二甲基丙烯酸酯（EGDMA）为交联剂,偶氮二异丁腈（AIBN）为引发剂,在紫外光的作用下合成RES分子印迹水凝胶膜（MIPH）。利用扫描电镜（SEM）考察光子晶体和MIPH的形貌特征,结果表明光子晶体排布规整,形成了六方堆积结构,MIPH具有三维（3-D）相互贯通的大孔结构。用UV测定光子晶体和MIPH的布拉格衍射吸收峰,MIPH对RES响应性良好,在3min内达到吸附平衡状态。在RES的不同浓度中（10-9¹0-5mol/L）,MIPH的布拉格衍射吸收峰从440nm红移到463nm。由于MIPH灵敏度高、响应速度快和特异性的识别模板分子等特点,使得MIPH在食品检测领域中具有广阔的应用前景。

Abstract: In order to detect the content of RES in samples rapidly and effectively, RES molecular imprinted photonic hydrogel ( MIPH ) was synthesized using photonic crystal as the template , RES as the template molecule , MAA as the functional monomer , EGDMA as the cross-linker and AIBN as the initiator . The morphology characterization of photonic crystal and MIPH was studied by scanning electron microscope (SEM) . The results showed that photonic crystal was formed by ordered silica developing into six party accumulation structures, and the 3-D interconnected macroporous structure was shaped in MIPH. The Bragg diffraction of photonic crystal and MIPH was investigated by ultraviolet spectrophotometer, and the MIPH after eluting RES was responsive to RES within 3 minutes, the Bragg diffraction peak shifted from 440 to 463nm with the concentration of RES increasing from 10-9to 10-5mol/L. Due to high sensitivity and specificity, quick response and easy operation, the application of MIPH in food detection area was promising.