Abstract: Methods of detection and determination of ergot alkaloids in grain and its products were reviewed.They included traditional detection methods:such as colorimetry and thin layer chromatography, instrumental procedures:such as liquid chromatography with fluorescence, mass spectrometric (MS) or MS/MS detection, capillary zone electrophoresis, immunological detection methods:such as radioimmunoassay, enzyme-linked immunosorbent assay (ELISA) . The research progress of ergot alkaloids analysis was summarized.