Abstract: Using carmine radish as raw material, the extraction technology of pigment from carmine radish by ultrasonic-assisted was optimized. Based on the single factor experiments, and used the response surface optimization experiments, and made the extraction yield as the index, the best technological conditions of ultrasonic-assisted method were determined. Under the conditions of the ethanol concentration 50% , liquid-solid ratio 42.6∶1, the extraction time 50min, extraction temperature was 70℃, pH4.6, the extraction yield was the maximum. Extraction yield of ultrasonic-assisted method was obviously higher than ethanol extracted method, and the extraction yield was 75.15%.