顶空固相微萃取结合气质联用分析沙葱中挥发性成分

Identification of volatile compounds in Allium Mongolicum Regel by head space solid phase micro-extraction coupled to gas chromatography-mass spectrometry (HS-SPME-GC-MS)

摘要: 沙葱是一种生长于荒漠地带的野生蔬菜,具有独特的气味。为了确定其挥发性成分,实验采用顶空固相微萃取技术（HS-SPME）对其挥发性成分进行萃取,然后用气相色谱质谱联用（GC-MS）进行检测。结果表明,共检测到24种化合物,在DVB/CAR/PDMS、PDMS/DVB、PA三种固相萃取头中,DVB/CAR/PDMS型号的萃取头萃取效果最佳;沙葱中主要挥发性成分是:2-己烯醛（27.74%）、甲基-2-烯丙基三硫醚（25.70%）、二甲基三硫化物（13.62%）、烯丙基甲基二硫醚（9.94%）、二烯丙基二硫（7.23%）,占总成分的84.23%,其中2-己烯醛在沙葱中首次报道。

Abstract: Allium Mongolicum Regel was a kind of wild vegetable which distributed in desert regions with unique odor. HS-SPME-GC-MS was applied to identify the volatile compounds in Allium Mongolicum Regel based on three solid phase fibers of DVB/CAR/PDM, PDMS/DVB and PA. Results showed that 24 compounds were identified and DVB/CAR/PDMS was the optimal one to extract more compounds. 2-Hexenal content was 27.74% , which was the most component, and other four compounds were Risulfide methyl 2-propenyl (25.70% ) , Dimethyl trisulfide (13.62% ) , and Disulfide methyl 2-propeny (7.23% ) , Diallyl disulphide (7.23% ) , which totally represented 84.23% of the identified compounds, 2-Hexenal was first reported in Allium Mongolicum Regel.