羊栖菜岩藻黄质色素的抗氧化性研究
Study on antioxidant activities of fucoxanthin pigments from Sargassum Fusiform
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摘要: 为研究羊栖菜超临界CO2提取的岩藻黄质色素粗品的抗氧化活性,首先应用分光光度法测定了岩藻黄质粗品的还原能力、对DPPH·清除率以及抗脂质过氧化功能,再通过化学发光法测定了岩藻黄质粗品清除·OH、O-2·和H2O2的性能。结果表明,羊栖菜岩藻黄质色素粗品浓度与抗氧化活性均呈良好的量效关系,对·OH、O-2·、DPPH·与H2O2具有较强的清除能力,还原能力表现较弱。其中岩藻黄质粗品·OH的IC50为386.29μg/mL,清除率强于对照品VC,弱于VE;DPPH·的IC50为565.2μg/mL,约为对照品VE的211.4%;O-2·的IC50为615.71μg/mL,H2O2的IC50为658.74μg/mL,清除率均小于VE、VC对照品。岩藻黄质粗品抗脂质过氧化功效为BHT的50%左右,远强于VC。因此,羊栖菜岩藻黄质色素粗品具有良好的抗氧化活性,有望成为天然抗氧化剂得到开发利用。Abstract: In order to study the antioxidant activities of fucoxanthin products extracted by supercritical carbon dioxide from Sargassum Fusiform, first, the experiments of reducing power, the clean-up rates on DPPH· and preventing effect on lipid peroxidation were performed using spectrophotometry and then the function of clean-up on ·OH, O-2·free radicals and H2O2 were studied by the method of chemiluminescence. Results indicated that fucoxanthin products of Sargassum Fusiform had doseresponse relationship with antioxidant activities, throwing a weak reducing power but strong clean-up rates on ·OH, O-2·free radicals, H2O2 and DPPH·. The IC50 for clean- up rates on ·OH was 386. 29μg/mL, superior to VC’s but below VE’s, the IC50 to DPPH· was 565. 2μg/mL, about 211. 4% of the VE’s and the IC50 to O-2· was 615. 71μg/mL, less than that of VE and VC. Preventing effect on lipid peroxidation was about 1/2 times of BHT and much more powerful than VC. Therefore, fucoxanthin products from Sargassum Fusiform had well antioxidant activities, and could be developed and used as native antioxidant hopefully.
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