Abstract: In order to reduce physicochemical properties of anthocyanin on its limitation of physiological activities, soy lecithin and cholesterol were used for membrane material on anthocyanin embedding. Anthocyanin liposome was prepared by rotary evaporated film ultrasonication method. The controlling index was determined by encapsulation efficiency of anthocyanin. The condition of preparation technology was optimized by single factor production and response surface. Preparation of anthocyanin optimal process was temperature 48. 80℃, lecithin to cholesterol mass ratio 1. 79∶ 1 (mg/mg) , anthocyanin content 0. 12mg, under this condition the entrapment efficiency was up to 74. 54%.