Abstract: Response surface methodology was applied to optimize the extraction conditions of carotenoids in Marigold.On the basis of single-factor test, the mathematical regression model was established about dependent variable (absorbance values in a specific wavelength of carotenoids, using ethyl acetate as solvent) and independent variables (ratio of solvent to material, extraction time and temperature) through Box-Benhnken center composite design and response surface analysis.The result showed that regression equation fit well with experimental data, the optimum extraction conditions of capsanthin were extraction time of 4.5h, extraction temperature of 45℃, material/liquid ratio of 1∶25 (g/mL) .Measured under optimal conditions, the average absorbance was 0.824 about carotenoids from Marigold extract.The optimized technology was stable and viable.