南昆山毛叶茶花色苷提取优化工艺研究

Study on optimizing conditions for anthocyanins extraction from Camellia ptilophylla chang

摘要: 为优化超声辅助提取南昆山毛叶茶紫芽花色苷的工艺,本研究对主要提取工艺参数进行三因素三水平实验设计,采用二次回归旋转组合实验,确定超声辅助提取优化条件。研究结果表明,在实验范围内,各因素对南昆山毛叶茶紫芽提取量影响程度从大到小依次为超声提取时间、超声功率、料液比,确定超声波提取的最佳工艺条件为超声时间25min,超声功率158W,料液比1:33(g/mL),在此条件下,花色苷提取量为8.092mg/g。

Abstract: In this experiment, optimization for anthocyanins extraction from purple leaves of Camellia ptilophylla chang was investigated using response surface methodology.The optimization strategy used was a three-factor three-level quadratic regression rotary combination design.The importance of three process parameters affecting extraction yield of anthocyanins in the order from highest to lowest was:ultrasonic treatment time, ultrasonic power, liquid-to-solid ratio.Ultrasonic treatment time of 25min, ultrasonic power 158W, liquid-to-solid ratio 1∶33 (g/mL) were found optimum for anthocyanins extraction from Camellia ptilophylla chang.Under the optimum technology, the extraction of anthocyanins was 8.092mg/g.