果糖月桂酸单酯的分离纯化及鉴定

Purification and identification of monolauroyl fructose

摘要: 采用硅胶柱层析色谱对叔戊醇中固定化磷脂酶Lecitase Ultra催化合成的果糖月桂酸单酯进行分离纯化。确定了薄层层析（TLC）条件,并通过反相-高效液相色谱-蒸发光散射检测（RP-HPLC-ELSD）测定柱层析纯化产物的纯度。利用液质联用（HPLC-ESI-MS）、红外光谱（FT-IR）和13C核磁共振（13CNMR）对目标产物进行确认,鉴定目标产物为酯化位点在1位和6位的果糖月桂酸单酯,四种异构体如下:1-月桂酸-β-D-吡喃果糖单酯、1-月桂酸-α-D-吡喃果糖单酯、1-月桂酸-β-D-呋喃果糖单酯和6-月桂酸-β-D-呋喃果糖单酯,且1-月桂酸-β-D-吡喃果糖单酯的比例最高。

Abstract: A novel enzymatic-catalyzing process of monolauroyl fructose was developed.In the process immobilized Lecitase Ultra was used as biocatalyst in anhydrous 2-methyl-2-butanol.Silica gel column chromatography was used to separate and purify the fructose ester.The qualitative analysis condition of TLC was confirmed and the purity was identified by RP-HPLC-ELSD.Structure was verified by HPLC-ESI-MS, FT-IR, 13C NMR.As a result, a mixture of the C-1 and C-6 monoacylated frutose esters, four isomers of monolauroyl fructose were obtained:l-lauroyl-β-D-fructopyranose, 1-lauroyl-α-D-fructopyranose, 1-lauroyl-β-D-fructofuranose, 6-lauroyl-β-D-fructofuranose, and l-lauroyl-β-D-fructopyranose was the highest proportion.