胆碱酯酶保藏方法的筛选及固定化研究

Study on cholinesterase preservation method of screening and immobilized

摘要: 目的:对胆碱酯酶进行保藏研究,以期得到最佳的酶活力。方法:以小麦酯酶为实验材料,采取冷藏、冷冻和酶的固定化的措施,添加不同种类和不同比例的防腐剂,采用不同的包装材料进行比较研究。结果:能够保持胆碱酯酶的酶活力方法是:设定保藏温度为-20℃,添加0.1%硫柳汞采用塑料瓶包装储藏,这样可以很好地保持酶液的酶活力。结论:采用低温冷冻保藏胆碱酯酶并且添加一定剂量的防腐剂可以很好的保持胆碱酯酶酶活力。

Abstract: Objective:Study the preservation methods about the CHE, in order to get the best enzyme activity.Methods:Based wheat on the testing material, took cold storage, frozen and enzyme immobilization measures, adding different types and different proportions of preservatives, using the different packing material were investigated.Results:The result showed that the better effect of remain well enzyme solution of the enzyme activity was set for-20℃ temperature preservation, adding 0.1% thimerosal used plastic bottles packaging storage.Conclusion:The cold preservation CHE and adding regular doses of preservative could make cholinesterase enzyme very good maintenance.