Aspergillus niger C15产柚苷酶发酵条件的优化研究

Study on the optimum fermentation conditions of Aspergillus niger C-15 for the production of naringinase

摘要: 通过正交实验对黑曲霉菌株C15产柚苷酶的发酵条件进行了优化。实验结果表明,Aspergillus niger C15菌株的最优产酶发酵条件为:2%桔皮粉、6%豆粕粉,250mL三角瓶中装料30mL,接种量3%,最适初始pH为5.0,30℃条件下培养100h,产酶量可达1395.9U/mL。与原始产酶条件相比,产酶量提高8.6%。不同的金属离子对产酶有不同的作用,添加适量的K2HPO4、MgSO4对产酶有明显的促进作用,CuSO4、FeSO4对产酶起抑制作用。

Abstract: The fermentation conditions for Aspergillus niger C15 naringinase production were optimalized by orthogonal experiment.The experimental result indicated that the best fermentation conditions was as follows:2% citrus peel meal and 6% soybean meal, 30mL culture media in 250mL beaker flask, 3% inoculation amount, initial pH 5, incubation temperature 30℃, time 100h.Under these optimized conditions, the yielding capacity of naringinase by the mutant was 1395.9U/mL, which was increased by 8.6% compared with the initial level (1285.6U/mL) .Proper quantity of K2HPO4 and MgSO4 could improve naringinase production, CuSO4, FeSO4 were bad for producing enzyme.