氨基酸与丙二醛反应体系的荧光光谱

doi:10.13386/j.issn1002-0306.2012.22.020

氨基酸与丙二醛反应体系的荧光光谱

Fluorescence spectroscopy of the reaction system between amino acid and malondialdehyde

摘要

摘要: 采用荧光光谱法研究氨基酸与丙二醛的反应,考察反应时间、pH和浓度对赖氨酸与丙二醛反应体系发射光谱的影响,并对不同种类的氨基酸与丙二醛反应进行比较。结果表明,氨基酸与丙二醛反应生成了400nm激发、460nm发射的荧光物质,该反应在中性条件下易于进行,并随时间延长产物增多,反应体系中,丙二醛浓度高于赖氨酸浓度有利于生成荧光产物。不同氨基酸与丙二醛反应产物的荧光强度差异明显,精氨酸、赖氨酸、甘氨酸、苯丙氨酸、组氨酸等五种氨基酸易与丙二醛反应,脯氨酸、羟脯氨酸、色氨酸未能与丙二醛反应生成荧光物质。

Abstract: The reaction between amino acid and malondialdehyde (MDA) was studied by fluorescence spectroscopy.The effect of time, pH and concentration on the emission spectroscopy of lysine-MDA system was researched, and the reaction charactertic of 19 kinds of amino acid with MDA was compared.The result showed the product of AA-MDA system had Ex.400nm and Em.460nm, easier to happen under the neutral condition, with the reaction time prolonged, the fluorescence product increased.The concentration of MDA was much more than that of lysine, more fluorescence product increasing.The fluorescence intensity had significant difference among kinds of AA-MDA.Arginine, lysine, histidine and glycine could easier react with MDA to produce fluorescence product, while proline, hydroxyproline and tryptophan could not.