Abstract: Objective:To select deproteinization-technology of polysaccharides in sugarcane leaf.Methods:These different ways to removing protein from polysaccharides in sugarcane leaf contain natural clarifying agents, Sevage method, TCA and TCA-Sevage method, and that were compared with the clearance rate of protein and the polysaccharide retention as indexes, and the technology was optimized by orthogonal experiment.Results:Type Ⅱ ZTC1 +1 natural clarifying agents could effectively purify sugarcane leaf polysaccharides, and the optimum condition of deproteinization with type Ⅱ ZTC1 +1 natural clarifying agents were as follows:the adding amount of natural clarifying agents (i.e B and A) were 6% and 3% respectively, the clarifying temperature was 30℃, concentration of polysaccharides was 3% (m/v) , the clarifying time (i.e B and A) were 1h and 1h respectively.Conclusion:TypeⅡZTC1+1 natural clarifying agents could effectively remove protein of sugarcane leaf polysaccharides, which was relatived to the amount of clarifying agents and temprature.