红皮云杉多酚的提取及其抗氧化活性研究

doi:10.13386/j.issn1002-0306.2012.20.031

红皮云杉多酚的提取及其抗氧化活性研究

Study on the extraction conditions and antioxidition of polyphenol from Picea Koraiensis Nakai

摘要

摘要: 以红皮云杉球果为原料,采用溶剂浸提法,通过单因素实验及正交实验,研究了料液比、乙醇浓度、提取温度及提取时间对红皮云杉球果多酚类化合物提取的影响,并考察了红皮云杉球果多酚的总抗氧化性、清除羟基自由基的能力、清除超氧阴离子的能力以及清除DPPH.的能力。结果表明,红皮云杉球果多酚的最佳提取工艺条件为:液料比25:1,乙醇浓度60%,提取温度60℃,提取时间3h,红皮云杉球果多酚提取量为136.3mg/g。在此条件下提取的红皮云杉球果多酚,对于ABTS+.、OH.、DPPH.和O2-.都具有较好的清除作用,且均强于VC和BHA。

Abstract: The paper utilized solvent to extract polyphenols from Picea Koraiensis Nakai pinecone.The effect of ratio of material to liquid, alcohol concentration, extraction temperature and extraction time on the polyphenol yield was researched by single factor experiment and orthogonal test.At the same time, the ability of total antioxidition, the ability to eliminating OH., DPPH.and O 2.were investigated.The result showed that the optimum extraction condition of polyphenols was as the following:the ration of liquid to material was 25:1, alcohol concentration was 60%, the extraction temperature was 60℃, the extraction time was 3h, the yield of polyphenols extraction from Picea Koraiensis Nakai pinecone was 136.3mg/g.The polyphenol extraction from Picea Koraiensis Nakai pinecone had good eliminating effect on ABTS +., OH., DPPH.and O 2., that was better than V C and BHA.