水产品中氯霉素、甲砜霉素和氟苯尼考的HPLC-UVD法测定

Determination of residues of chloramphenicol, thiamphnicol and florfenicol in fishery products by HPLC-UVD

摘要: 建立同时检测水产品中氯霉素、甲砜霉素和氟苯尼考的HPLC-UVD法。以2%碱性乙酸乙酯作为提取溶剂,正己烷脱脂后直接经高效液相色谱进行分析。氯霉素、甲砜霉素和氟苯尼考在0.10～10.00mg/L范围内有良好的线性关系。在添加浓度为0.25～5.00mg/kg时,平均回收率在81.00%～102.93%之间,RSD在1.95%～7.10%之间。氯霉素的检出限为0.01mg/kg,甲砜霉素和氟苯尼考的检出限为0.02mg/kg。

Abstract: A high-performance liquid chromatography with ultraviolet detection method was developed for simultaneous determination of Chloramphenicol (CAP) , Thiamphnicol (TAP) and Florfenicol (FF) in fishery products. Chloramphenicols (CAPs) residues were extracted by basic ethyl acetate , followed by sample cleanup with n-hexane and quantization by liquid chromatography with ultraviolet detector. Good linearities were obtained in the concentration ranging from 0.10 ～10.00mg/L for CAP, TAP and FF. The average recoveries and relative standard deviations (RSDs) for the analysis of all samples fortified over the range of 0.25～5.00mg/kg were in the range of 81.00%～102.93% and 1.95%～7.10%, respectively. The limits of detection were 0.01mg/kg for CAP and 0.02mg/kg for TAP and FF.